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Key Documents

MAB3083

Sigma-Aldrich

Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1

ascites fluid, clone P1, Chemicon®

Synonym(s):

Anti-CALPAIN4, Anti-CANP, Anti-CANPS, Anti-CAPN4, Anti-CDPS, Anti-CSS1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

P1, monoclonal

species reactivity

human, bovine

should not react with

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001003962.1
NM_001749.2

UniProt accession no.

P04632

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CAPNS1(826)

General description

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Specificity

Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).

Immunogen

Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)

Application

Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D Bano et al.
Cell death and differentiation, 17(1), 119-133 (2009-08-29)
Cell death requires coordinated intracellular signalling before disassembly of cell architecture by degradative enzymes. Although the death signalling cascades that involve the mitochondria, the ER and the plasma membrane have been extensively characterized, only a handful of studies have examined
Estrogen administration attenuates immobilization-induced skeletal muscle atrophy in male rats.
Sugiura, T; Ito, N; Goto, K; Naito, H; Yoshioka, T; Powers, SK
The Journal of Physiological Sciences : Jps null
R L Mellgren et al.
Biochimica et biophysica acta, 954(2), 154-160 (1988-05-18)
Calpains (EC 3.4.22.17) are nonlysosomal intracellular proteinases which require calcium ion for activity. The calpains are heterodimers composed of a large catalytic subunit and a small subunit which may have a regulatory function during the catalytic cycle. However, whether calpains
Critical role for cathepsin B in mediating caspase-1-dependent interleukin-18 maturation and caspase-1-independent necrosis triggered by the microbial toxin nigericin.
Hentze, H; Lin, XY; Choi, MS; Porter, AG
Cell Death and Differentiation null
A radioimmunologic technique for assessing calpain activation in cells.
R L Mellgren
Methods in molecular biology (Clifton, N.J.), 144, 161-169 (2000-05-20)
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