HTS144M
ChemiSCREEN Membrane Preparation Recombinant Human NK2 Tachykinin Receptor
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41123201
eCl@ss:
32161000
NACRES:
NA.45
description
GPCR Class A
protein target NK2
Quality Level
technique(s)
radioligand binding assay (RLBA): suitable
UniProt accession no.
Gene Information
human ... TACR2(6865)
General description
FUNCTION: SwissProt: P21452 # This is a receptor for the tachykinin neuropeptide substance K (neurokinin A). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 398 amino acids; 44439 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P21452 ## Belongs to the G-protein coupled receptor 1 family.
MISCELLANEOUS: The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P.
SIZE: 398 amino acids; 44439 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P21452 ## Belongs to the G-protein coupled receptor 1 family.
MISCELLANEOUS: The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P.
Application
Radioligand binding assay and GTPγS binding
Physical form
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Storage and Stability
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Analysis Note
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA, containing one Protease Inhibitor Cocktail Tablet (Roche Cat. No. 11 873 580 001) for each 50ml binding buffer.
Radioligand: [125I]-NKA (NEX 252)
Wash Buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled NKA at 0.5nM.
Binding buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA, containing one Protease Inhibitor Cocktail Tablet (Roche Cat. No. 11 873 580 001) for each 50ml binding buffer.
Radioligand: [125I]-NKA (NEX 252)
Wash Buffer: 50mM Tris, pH 7.4, 150mM NaCl, 3mM MnCl2, 0.1% BSA
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled NKA at 0.5nM.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service