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AB3081

Sigma-Aldrich

Anti-Na+/H+ Exchanger-1 Antibody

Chemicon®, from rabbit

Synonym(s):

NHE1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, bovine, mouse, pig

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_003047.2

UniProt accession no.

P19634

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC9A1(6548)

Specificity

Recognizes Na+/H+ Exchanger 1 (NHE1). The immunogen peptide shows no significant homology with other NHE isoforms (NHE2-5).

Immunogen

A 22 aa peptide within the cytoplasmic, C-terminal domain of rat NHE1, coupled to KLH.

Application

Detect Na+/H+ Exchanger-1 using this Anti-Na+/H+ Exchanger-1 Antibody validated for use in ELISA & WB.
Western blot: 1-10 μg/mL using Chemiluminescence technique.



ELISA: 1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by end user.

Target description

91 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS and 0.1% BSA .

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Malignant gliomas display altered plasma membrane potential and pH regulation--interaction with Tc-99m-MIBI and Tc-99m-Tetrofosmin uptakes.
N Perek, D Denoyer, F Dubois, F Koumanov
General Physiology and Biophysics null
Bryan J Feger et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 305(10), R1182-R1189 (2013-09-21)
The myocardial Na(+)/H(+) exchanger-1 (NHE1) plays a major role in regulation of intracellular pH, and its upregulation has been implicated in increased ischemia-reperfusion injury and other pathologies. Hydrogen peroxide (H2O2) increases NHE1 activity acutely via ERK1/2 signaling. Chronic strenuous exercise
J Orlowski et al.
The Journal of biological chemistry, 267(13), 9331-9339 (1992-05-05)
Biochemical and pharmacological data support the existence of multiple forms of the Na/H exchanger (NHE). Two isoforms, termed NHE-1 and NHE-2, have recently been isolated from rabbit ileal villus epithelial cells (Tse, C. M., Ma, A. I., Yang, V. W.
Danny Christiansen et al.
Acta physiologica (Oxford, England), 231(3), e13580-e13580 (2020-11-23)
To assess how blood-flow-restricted (BFR) interval-training affects the capacity of the leg muscles for pH regulation during dynamic exercise in physically trained men. Ten men (age: 25 ± 4y; V˙O2max : 50 ± 5 mL∙kg-1 ∙min-1 ) completed a 6-wk interval-cycling intervention (INT) with one
C H Yun et al.
The American journal of physiology, 269(1 Pt 1), G1-11 (1995-07-01)
Na+/H+ exchangers are integral plasma membrane proteins that exchange extracellular Na+ for intracellular H+ with a stoichiometry of one for one. They are inhibitable by the diuretic amiloride and have multiple cellular functions, including intracellular pH homeostasis, cell volume control
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