481480

NF-κB SN50, Cell-Permeable Inhibitor Peptide

NF-κB SN50, CAS 213546-43-3, is a cell-permeable peptide that contains the NLS sequence of NF-κB p50 linked to the hydrophobic region of K-FGF. Inhibits translocation of the NF-κB into the nucleus.

• Synonym(s): NF-κB SN50, Cell-Permeable Inhibitor Peptide
• Empirical Formula (Hill Notation): C₁₂₉H₂₃₀N₃₆O₂₉S
• Molecular Weight: 2781.50
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Contains the nuclear localization sequence (NLS) of the transcription factor NF-κB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). The N-terminal K-FGF h-region confers cell-permeability, while the NLS (360-369) inhibits translocation of the NF-κB active complex into the nucleus. In murine endothelial LE-II cells induced by LPS, NF-κB nuclear translocation is maximally inhibited at 18 µM.

Contains the nuclear localization sequence (NLS) of the transcription factor NF-κB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). The peptide N-terminal K-FGF h-region confers cell-permeability, while the NLS (360-369) inhibits translocation of the NF-κB active complex into the nucleus. In murine endothelial LE-II cells induced by LPS, inhibition of NF-κB nuclear translocation is maximally inhibited at 18 µM.

NF-κB SN50, CAS 213546-43-3, is a cell-permeable peptide that contains the NLS sequence of NF-κB p50 linked to the hydrophobic region of K-FGF. Inhibits translocation of the NF-κB into the nucleus.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
translocation of the NF-κB active complex into the nucleus

Product does not compete with ATP.

Warning

Toxicity: Standard Handling (A)

Sequence

H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Other Notes

Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.

Legal Information

Sold under license of U.S. Patents 5,807,746 and 6,043,339 for research use only. Licenses for commercial manufacture, commercial use, diagnostics, or therapeutics may be obtained by contacting Vanderbilt University, Nashville, Tennessee.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany