07-1232
Anti-Notch 1 Antibody, NT
serum, from rabbit
Synonym(s):
Neurogenic locus notch homolog protein 1 precursor, Notch (Drosophila) homolog 1 (translocation-associated), Notch 1, Notch homolog 1, translocation-associated (Drosophila), Translocation-associated notch protein TAN-1, Neurogenic locus notch homolog pro
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
species reactivity (predicted by homology)
rat (immunogen homology)
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NOTCH1(4851)
General description
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
Specificity
Notch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
Other species have not been tested.
Immunogen
Epitope: Cleaved N-Terminus
Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1.
Application
Anti-Notch 1 Antibody, N-Terminus is a Rabbit Polyclonal Antibody for detection of Notch 1 also known as Neurogenic locus notch homolog protein 1 precursor & has been validated in ELISA, WB, IHC(P).
ELISA:
A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine Cancer
ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine Cancer
ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates.
Target description
~80 kDa
Linkage
Replaces: 04-1046
Physical form
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.1% sodium azide.
Storage and Stability
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues.
Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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