06-1038
Anti-Unc84B/SUN2 Antibody
0.5 mg/mL, from rabbit
Synonym(s):
Rab5-interacting protein, Sad1 unc-84 domain protein 2, Sad1/unc-84 protein-like 2, nuclear envelope protein, unc-84 homolog B, unc-84 homolog B (C. elegans)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, canine
species reactivity (predicted by homology)
mouse (87%), dog, horse, rat (87%)
concentration
0.5 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SUN2(25777)
General description
UNC84B (Protein UNC-84 homolog B or SUN2), is a single-pass nuclear envelope transmembrane protein responsible for nuclear migrations that are essential for development. UNC-84B has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. UNC-84B interacts with RAB5A and is widely expressed, and highly expressed in the heart, lungs and muscles. UNC-84 may function to facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage.
Specificity
This antibody recognizes Unc84B/SUN2 at the N-terminus.
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human Unc84B/SUN2 at and around the N-terminus.
Application
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Unc84B/SUN2 from 500 µg of U2OS cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
This Anti-Unc84B/SUN2 Antibody is validated for use in Immunoprecipitation and Western Blotting for the detection of Unc84B/SUN2.
Quality
Evaluated by Western Blotting in U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Target description
~80 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U2OS cell lysate.
U2OS cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture.
Korfali, N; Wilkie, GS; Swanson, SK; Srsen, V; Batrakou, DG; Fairley, EA; Malik et al.
Molecular and Cellular Proteomics null
Nadia Korfali et al.
Methods in molecular biology (Clifton, N.J.), 1411, 3-44 (2016-05-06)
Nuclei can be relatively easily extracted from homogenized liver due to the softness of the tissue and crudely separated from other cellular organelles by low-speed centrifugation due to the comparatively large size of nuclei. However, further purification is complicated by
Nanami Ueda et al.
Frontiers in cell and developmental biology, 10, 885859-885859 (2022-06-07)
The linker of nucleoskeleton and cytoskeleton (LINC) complex is composed of the inner nuclear membrane-spanning SUN proteins and the outer nuclear membrane-spanning nesprin proteins. The LINC complex physically connects the nucleus and plasma membrane via the actin cytoskeleton to perform
Nikolaj Zuleger et al.
Genome biology, 14(2), R14-R14 (2013-02-19)
Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells
Nadia Korfali et al.
Nucleus (Austin, Tex.), 3(6), 552-564 (2012-09-20)
One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle.
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