G005822

GC Analysis of FAMEs in Peanuts on Omegawax^™

suitable for GC

Properties

• technique(s): gas chromatography (GC): suitable
• test parameters: column: OMEGAWAX 250, 30 m x 0.25 mm I.D., 0.25 μm (24136); oven: 170 °C, 1 °C/min to 225 °C; inj. temp.: 250 °C; detector: FID, 250 °C; carrier gas: helium, 1.2 mL/min constant flow; injection: 1 μL, split 100:1; liner: 4 mm ID FocusLiner w/taper; sample: Dry roasted, unsalted peanuts prepared for analysis per AOCS Official Method Ce 1k-09
• application(s): agriculture; food and beverages

Description

Analysis Note

This application illustrates the extraction and GC analysis of peanuts for fatty acids. The health benefits of peanuts and peanut oil are associated with their high oleic acid content. Oleic acid is believed to help lower cholesterol and reduce the risk of heart disease. Oleic acid, also found in olive oil, is a major contributor to the health benefits associated with the “Mediterranean Diet.”
The samples were prepared using acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09. BHT was added as an antioxidant prior to extraction. All samples were concentrated to 1 mL prior to GC analysis. The Omegawax GC column was used in the analysis of the saturated and unsaturated fatty acids present in the sample.

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