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G004529

HPLC Analysis of Dopa and Carbidopa Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 20 mM ammonium acetate, pH 4; [B] methanol; (30:70, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 5 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

clinical

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description

    3,4-Dihydroxy-L-phenylalanine, ≥98% (TLC)
    S-(−)-Carbidopa, ≥98% (TLC), powder
    3,4-Dihydroxy-D-phenylalanine, powder, ≥95%

standard


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