ZRB2290
Anti-MAP2 Antibody, clone 3C19, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Microtubule-associated protein 2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3C19, monoclonal
recombinant monoclonal
description
3C19 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 199.53 kDa
observed mol wt ~260 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MAP2(4133)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 3C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Microtubule-associated protein 2. It targets an epitope with in 17 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Microtubule-associated protein 2 (MAP2).
Application
Anti-MAP2, clone 3C19 ZooMAb, Cat. No. ZRB2290, is a recombinant Rabbit Monoclonal Antibody that specifically targets Microtubule-associated protein 2 (MAP2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAP2 in mouse brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in E18 cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MAP2 in rat brain, mouse brain, human brain and in rat kidney tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in E18 cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MAP2 in rat brain, mouse brain, human brain and in rat kidney tissue sections.
Target description
Microtubule-associated protein 2 (UniProt: P11137; also known as MAP-2) is encoded by the MAP2 gene (Gene ID: 4133) in human. MAPs are a family of thermostable cytoskeletal proteins that bind to and stabilize microtubules. MAP4 is expressed ubiquitously while MAP1 and MAP2 are expressed primarily in neurons. MAP2 is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. In the central nervous system, MAP2 is confined to neuronal cell bodies and dendrites. However, some axons may also have small amounts of MAP2. It is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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