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General description
The TargeTron Vector pNL9164 is a 9,295 bp expression vector for use with the TargeTron Gene Knockout System (product number TA0100). This circularized vector can be used for targeted gene knockouts in gram-positive organisms such as Staphyloccus aureus. As supplied, the pNL9164 vector is re-targeted to the S. aureus hsa gene. The hsa gene encodes a member of the HU family of bacterial histone-like proteins. For validation in S. aureus, this vector can be used directly to knockout hsa without any further modifications.
Legal Information
TargeTron is a trademark of InGex, LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Megan R Kiedrowski et al.
PloS one, 6(11), e26714-e26714 (2011-11-19)
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging contributor to biofilm-related infections. We recently reported that strains lacking sigma factor B (sigB) in the USA300 lineage of CA-MRSA are unable to develop a biofilm. Interestingly, when spent media from a
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