T0678

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse

clone WH-3, ascites fluid

• Synonym(s): Anti-TPH
• MDL number: MFCD00282500
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: WH-3, monoclonal
• mol wt: antigen 55 kDa
• contains: 15 mM sodium azide
• species reactivity: human, monkey, rabbit, rat
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:1,000 using rabbit pineal gland extract
• isotype: IgG3
• UniProt accession no.: P17752
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TPH1(7166) , TPH2(121278) ; rat ... Tph1(24848) , Tph2(317675)

Description

General description

Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.

The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.

Specificity

This antibody reacts specifically with tryptophan hydroxylase.

Immunogen

recombinant rabbit tryptophan hydroxylase.

Application

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunoblot
• immunocytochemistry

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable