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RAB0654

Sigma-Aldrich

Human EPO / Erythropoietin ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Erythropoietin

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.15 mIU/mL
standard curve range: 0.137-100 mIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EPO(2056)

Related Categories

General description

The antibody pair provided in this kit recognizes Human Erythropoietin.

Application

Human EPO / Erythropoietin ELISA Kit has been used to measure concentration of erythropoietin (EPO).
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Erythropoietin (EPO) plays a vital role in the regulation of red blood cell production. It interacts with EPO receptor and is responsible for the cellular responses. EPO is expressed in various types of cancers, such as breast cancer, renal cancer, gastric cancer, hepatocellular carcinoma and central nervous system tumors. EPO also acts as a hypoxia responsive cytokine and a pro-angiogenic factor. This protein provides major neuroprotective effect against brain damages due to ischemia and enhances memory as well as cognitive ability in humans suffering from vascular dementia.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aspergillus niger has been employed to produce heterologous proteins due to its high capacity for expression and secretion; nevertheless, expression levels of human proteins have been modest. We were interested in investigating whether A. niger can express and secret human
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