biological source
bovine pancreas
Quality Level
form
liquid
specific activity
1500 units/mL
3000 units/mL
concentration
20 mg/mL in Tris buffer
technique(s)
DNA purification: suitable
storage temp.
room temp
Gene Information
bovine ... RNASE1(282340) , RNASE1(280930)
General description
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Application
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Biochem/physiol Actions
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
Preparation Note
For usage requirements, please refer to the kit procedure.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
sample prep
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
Singh A, et al.
PloS one, 13(10), e0206667-e0206667 (2018)
Abdou ElSharawy et al.
Oncotarget, 7(46), 75353-75365 (2016-09-30)
The emerging potential of miRNAs as biomarkers for cancer detection demands parallel evaluation of strategies for reliable identification of disease-related signatures from easily accessible and pertinent body compartments. Here, we addressed whether efficient concentration of circulating miRNA-carrying particles is a
Phenotypic plasticity and biogeographic variation in physiology of habitat-forming seaweed: response to temperature and nitrate
Flukes EB, et al.
Journal of Phycology, 51(5), 896-909 (2015)
Lenka Senohrabkova et al.
Current genetics, 65(4), 919-940 (2019-02-05)
Cells have elaborated a complex strategy to maintain protein homeostasis under physiological as well as stress conditions with the aim to ensure the smooth functioning of vital processes and producing healthy offspring. Impairment of one of the most important processes
Shiwen Zhang et al.
International journal of nanomedicine, 9, 1931-1946 (2014-05-03)
Gold nanorods (AuNRs) have been used in plasmonic photothermal therapy (PPTT), which is thought to be more efficient and selective than conventional photothermal therapy. The efficiency and safety of PPTT can be improved by functionally modifying the gold nanorods with
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service