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PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Synonym(s):

Phagocytic cell label

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

Quality Level

Related Categories

Application

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principle

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description

  • Diluent B 6 x 10

  • PKH26 cell linker in ethanol .5 mL

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup


Certificates of Analysis (COA)

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