P0609

Pepsin−Agarose from porcine gastric mucosa

lyophilized powder, ≥30 units/mg dry solid

• Synonym(s): Pepsin A
• Enzyme Commission number: 3.4.23.1 (BRENDA, IUBMB)
• MDL number: MFCD00081840
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Porcine gastric mucosa
• form: lyophilized powder
• specific activity: ≥30 units/mg dry solid
• mol wt: 35 kDa
• matrix: 4% cross-linked beaded agarose
• UniProt accession no.: P00791
• storage temp.: −20°C
• Gene Information: pig ... LOC396892(396892)

Description

General description

Pepsin is an enzyme found in gastric secretion.

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Biochem/physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Unit Definition

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Quantity

One gram solid swells to 5-10ml packed gel.

Physical form

Lyophilized powder stabilized with lactose

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves