L5142
Lectin from Triticum vulgaris (wheat)
biotin conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
UNSPSC Code:
12352202
eCl@ss:
34058011
NACRES:
NA.32
Recommended Products
conjugate
biotin conjugate
Quality Level
form
lyophilized powder
composition
Protein, ≥80% E1%/280
extent of labeling
2-4 mol biotin per mol protein
storage temp.
2-8°C
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General description
Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- in lectin binding assay of samples from intrauterine stages of filarial parasites
- in the histological studies of mice myocytes
- for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate
Preparation Note
Conjugates are prepared from affinity purified lectin.
Legal Information
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Use of lectin-magnetic separation (LMS) for detecting Toxoplasma gondii oocysts in environmental water samples
Harito JB, et al.
Water Research, 127, 68-76 (2017)
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)
A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)
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