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GE28-9909-46

Sigma-Aldrich

Superdex® 200 Increase 3.2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
titanium frit

product line

Superdex®

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

10000-600000 Da (globular proteins)

packaging

1 ea of

parameter

<0.15 ml/min flow rate
3.0 Mpa (652 psi)
4-50 mL sample volume

bed volume

~2.4 mL

column L × I.D.

30 cm × 3.2 mm

matrix

agarose-dextran composite
polymeric

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

Related Categories

General description

Superdex 200 is a column for high resolution gel filtration in micro scale (μg) preparative purification as well as characterization and analysis of small sample volumes (4-50 μL) of proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Publishing

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification as well as characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
for both preparative and analytical purposes

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Protocols

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Related Content

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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