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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

Synonym(s):

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

glutathione

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 17-0756-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)

Related Categories

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xin Zhou et al.
Nature communications, 11(1), 2674-2674 (2020-05-31)
Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and
Amandine Cartier-Michaud et al.
PLoS genetics, 13(6), e1006803-e1006803 (2017-06-16)
Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity
Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell
Amandine Draime et al.
FEBS letters, 592(7), 1185-1201 (2018-02-22)
HOXA1 belongs to the HOX family of transcription factors which are key regulators of animal development. Little is known about the molecular pathways controlling HOXA1. Recent data from our group revealed distinct partner proteins interacting with HOXA1. Among them, OGT
Amandine Draime et al.
Biochimica et biophysica acta, 1861(5), 534-542 (2018-02-23)
Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently
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Articles

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

General Considerations for Purification of GST-tagged Protein

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

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Protocols

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

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