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F0392

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Bovine Sera, Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
eCl@ss:
32160409

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Select

form

liquid

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used as a supplement for various cell culture techniques as it provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is a constituent of animal cell culture media. It contains hormones and growth factors. FBS promotes in vitro cell culture, by providing binding proteins, lipids, minerals, attachment and spreading factors.

Preparation Note

10,000 Molecular Weight Cut-off

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Blocking anaplerotic entry of glutamine into the TCA cycle sensitizes K-Ras mutant cancer cells to cytotoxic drugs
Saqcena M, et al.
Oncogene, 34(20), 2672-2672 (2015)
Stimulatory effect of luteinizing hormone, insulin-like growth factor-1, and epidermal growth factor on vascular endothelial growth factor production in cultured bubaline luteal cells
Chouhan VS, et al.
Theriogenology, 84(7), 1185-1196 (2015)
Liver Tissue Engineering Based on Poly (ethylen Glycol) Hydrogels (2010)
The use of fetal bovine serum: ethical or scientific problem?
Jochems CEA, et al.
Alternatives to Laboratory Animals : ATLA, 30(2), 219-227 (2002)
Direct interactions with both p27 and Cdk2 regulate Spy1-mediated proliferation in vivo and in vitro
Al Sorkhy M, et al.
Cell Cycle, 15(1), 128-136 (2016)
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Articles

Choosing the optimal cell culture media for vaccine production

Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

Serum for Cell and Tissue Culture FAQs

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