E2279

Monoclonal Anti-Endonuclease VIII antibody produced in mouse

~1.5 mg/mL, clone E8-122, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Endo VIII
• MDL number: MFCD03792417
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: E8-122, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~30 kDa by SDS-PAGE
• species reactivity: E. coli
• concentration: ~1.5 mg/mL
• technique(s): indirect ELISA: suitable; western blot: 10-20 μg/mL using recombinant endonuclease VIII (Cat No. E0651)
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti- Endonuclease VIII (mouse IgM isotype) is derived from the E8-122 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice . Endonuclease VIII (Endo VIII) from E. coli is a product of the nei gene. It is a DNA N-glycosylase and abasic site lyase (AP1 lyase. It comprises C-terminal Cys4-type β/β-antiparallel zinc finger with an arginine residue (Arg-252).

Specificity

Monoclonal Anti-Endonuclease VIII recognizes endonuclease VIII from E. coli (approx. 30 kDa).

Immunogen

recombinant full length endonuclease VIII from Escherichia coli.

Application

Monoclonal Anti-Endonuclease VIII antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Endonuclease VIII (Endo VIII) from E. coli is a DNA repair protein that recognizes and removes modified pyrimidines, such as thymine glycol (Tg) and 5,6,dihydrothymine (DHT), from DNA.. Its modified base substrate specificity overlaps the endonuclease III (endo III, nth) substrate specificity. This cross-reactivity is manifested in E. coli mutants. E. coli nth and nei mutants are either not sensitive or are slightly more sensitive to ionizing radiation and hydrogen peroxides than the wild type. The nei-nth double mutant is hypersensitive to oxidative stress. The mechanism used by Endo VIII to cleave the DNA backbone (lyase activity) is a β-δ elimination, resembling the action of formamidopyrimidine-DNA glycosylase (fpg) protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.