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DFF100

Sigma-Aldrich

DEAE–Sepharose™

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Synonym(s):

Diethylaminoethyl–Sepharose™

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About This Item

CAS Number:
57407-08-6
MDL number:
MFCD00146630
UNSPSC Code:
47101511
NACRES:
NA.56

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

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General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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