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D0547

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
22184-U22185-U20733
Hamilton® syringe, 7000 series, knurled hub 7105, volume 5 μL, needle size 24 ga (bevel tip), needle L 70 mm (2.75 in.)

20980-U

Hamilton® syringe, 7000 series, knurled hub

Hamilton® syringe, 7000 series, knurled hub 7000.5, volume 0.5 μL, needle size 25 ga (blunt tip), needle L 70 mm (2.75 in.)

22184-U

Hamilton® syringe, 7000 series, knurled hub

Hamilton® syringe, 7000 series, knurled hub 7000.5, volume 0.5 μL, needle size 25 ga (bevel tip), needle L 70 mm (2.75 in.)

22185-U

Hamilton® syringe, 7000 series, knurled hub

Hamilton® syringe, 7000 series, knurled hub 7101, volume 1 μL, needle size 22s ga (blunt tip), needle L 70 mm (2.75 in.)

20733

Hamilton® syringe, 7000 series, knurled hub

packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

needle size

24 ga (bevel tip)

needle size

25 ga (blunt tip)

needle size

25 ga (bevel tip)

needle size

22s ga (blunt tip)

feature

Sample contained in needle (no dead volume)

feature

no dead volume, sample contained in needle

feature

Sample contained in needle (no dead volume)

feature

Sample contained in needle (no dead volume)

manufacturer/tradename

Hamilton 88011

manufacturer/tradename

Hamilton 86250

manufacturer/tradename

Hamilton 86259

manufacturer/tradename

Hamilton 86200

needle L

70 mm (2.75 in.)

needle L

70 mm (2.75 in.)

needle L

70 mm (2.75 in.)

needle L

70 mm (2.75 in.)

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos[1]
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants[2]
  • to culture human corneal epithelial cell lines[3]

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantity

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

also commonly purchased with this product

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Description
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supplement

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF5955
RNBJ0236
SLBV3960
SLBV4752
RNBG0066

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Collagen and chondroitin sulfate (CS) are an essential component of the natural extracellular matrix (ECM) of most tissues. They provide the mechanical stability to cone the compressive forces in ECM. In tissue engineering, electrospun nanofibrous scaffolds prepared by electrospinning technique
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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