biological source
human esophagus (lung metastasis)
growth mode
Adherent
karyotype
Hypodiploid 39-41 chromosomes
morphology
Multicellular floating spheroids some with fibroblastoid morphology
products
P53 mutation c.164delC c.743G>A
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Barretts adenocarcinoma, adenocarcinoma of distal oesophagus
Application
Recommended culture medium: RPMI 1640 + 2mM L-Glutamine + 10% FBS
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20
Culture Medium
RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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