C2780

Chondroitinase AC from Flavobacterium heparinum

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

• Synonym(s): Chondroitin AC lyase
• CAS Number: 9047-57-8
• Enzyme Commission number: 4.2.2.5 (BRENDA, IUBMB)
• MDL number: MFCD00130796
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• conjugate: (Glucosaminoglycan)
• Quality Level: 200
• form: lyophilized powder
• specific activity: 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
• composition: Protein, ~15% Lowry
• solubility: 0.02 M phosphate buffer: soluble (pH 7.0)
• foreign activity: Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
• storage temp.: −20°C

Description

Application

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Biochem/physiol Actions

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Other Notes

Contains potassium phosphate buffer salts and BSA as stabilizer.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Suitability

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Unit Definition

One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

Preparation Note

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves