A6535
Apyrase from potatoes
ATPase ≥200 units/mg protein, lyophilized powder
Synonym(s):
Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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About This Item
type
Grade VII
Quality Level
form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
Protein, 25-60%
solubility
H2O: soluble 1.0 mg/mL
application(s)
diagnostic assay manufacturing
foreign activity
Acid Phosphatase ≤2% of base activity
storage temp.
−20°C
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Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.
Reaction: ATP → ADP+Pi → AMP+2Pi.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Other Notes
This grade has a low ATPase/ADPase ratio.
Unit Definition
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Physical form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note
Derived from red potato
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Properties of Two Apyrases from Solanum tuberosum
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