A0924

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 49:1 (ratio)

• Synonym(s): Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution
• MDL number: MFCD00080848
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770639
• NACRES: NA.25

Properties

• grade: for molecular biology
• product line: BioReagent
• form: powder
• feed ratio: 49:1 (ratio)
• foreign activity: DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

Application

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Precautionary Statements: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves