CMC0012
XLDNA SIG10 Electrocompetent cells
for BAC & cosmid cloning
About This Item
Recommended Products
biological source
Escherichia coli
grade
for molecular biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation
Genotype
F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)
Features and Benefits
- Cells that enable the most challenging library construction and screening.
- Highest available transformation efficiencies for library construction and screening.
- Optimized for high yields of DNA
Components
- XLDNA SIG10 electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
related product
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
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