94506
Tetramethylrhodamine-5-maleimide
suitable for fluorescence, ≥85% (HPLC)
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About This Item
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Quality Level
Assay
≥85% (HPLC)
form
powder
solubility
DMSO: soluble
methanol: soluble
fluorescence
λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
suitability
suitable for fluorescence
storage temp.
−20°C
InChI
1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)
InChI key
KGFLZYXDJDOIEE-UHFFFAOYSA-N
Application
Tetramethylrhodamine-5-maleimide has been used:
- to concentrate the eluate and in labeling to create IRE1LDS112C
- to derivatize green fluorescent protein (GFP) on Cys48 for experiments with the extrinsic fluorescent probe
- to incubate the single solvent exposed cys mutant to perform protein labeling
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Labeling reagent for protein thiols for cytofluorescence
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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