82452
Proteinase K, immobilized on Eupergit® C from Tritirachium album
powder (granular), ≥1500 U/g
Synonym(s):
Endopeptidase K, immobilized on Eupergit® C
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About This Item
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biological source
fungus (Tritirachium album)
form
powder (granular)
specific activity
≥1500 U/g
storage temp.
2-8°C
General description
Proteinase K is secreted extracellular medium by mold, Tritirachium album. It hydrolyses keratin and shows substrate specificity similar to serine alkaline proteinases.
Application
Proteinase K, immobilized on Eupergit® C from Tritirachium album has been used:
- in the protolysis of human serum
- in the digestion of gruel samples for enzyme linked immune assays (ELISA)
- in proteolytic stability studies of calcium ion flux inducers
Biochem/physiol Actions
Proteinase K hydrolyses keratin by cleaving peptide bonds adjacent to the carboxyl group of aliphatic and aromatic amino acids.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-tyrosine-ethylester (ATEE) per minute at pH 9.0 and 30 °C.
Legal Information
Eupergit is a registered trademark of Röhm GmbH & Co. KG
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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