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biological source
mouse
Quality Level
100
500
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
8G7, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (406M-14)
vial of 0.5 mL concentrate (406M-15)
bottle of 1.0 mL predilute (406M-17)
vial of 1.0 mL concentrate (406M-16)
bottle of 7.0 mL predilute (406M-18)
manufacturer/tradename
Cell Marque™
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50
isotype
IgG1κ
control
colon, colorectal adenocarcinoma, pancreatic ductal adenocarcinoma
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
General description
Mucin 4 (MUC4) is a transmembranous glycoprotein. MUC4 is expressed in the cytoplasm of certain epithelial surfaces, such as colonic, breast, and lung epithelia. An abnormal expression of MUC4 has been reported in various carcinomas of the colon, pancreas, breast, and ovaries.MUC4 is highly expressed in the vast majority of human pancreatic neoplasms, such as intraductal papillary mucinous neoplasia of the pancreas, pancreatic intraepithelial neoplasms and pancreatic ductal adenocarcinoma; however, it is not detected in the normal pancreas or in chronic pancreatitis.MUC4 is not expressed in normal bile ducts, but it can be overexpressed in intrahepatic cholangiocarcinoma and bile duct cholangiocarcinoma. MUC4 overexpression has been reported in low-grade fibromyxoid sarcoma (LGFMS). In this study a large cohort of LGFMS, all with FUS gene rearrangement confirmed by FISH, showed cytoplasmic staining for MUC4, usually in a strong and diffuse manner. Similarly, strong, diffuse cytoplasmic staining for MUC4 was identified in 78% (32/41) of cases of sclerosing epithelioid fibrosarcoma. MUC4 expression is also detected in the glandular component of biphasic synovial sarcomas (90%). Focal staining, usually interpreted as only scattered cells, was also seen in a subset of ossifying fibromyxoid tumors (29%), epithelioid GISTs (20%) and myoepithelial carcinomas (10%); whereas all other epithelioid soft tissue tumors—including clear cell sarcoma, epithelioid sarcoma, epithelioid hemangiosarcoma, PEComa and melanoma—were negative. It should be noted that various carcinomas may express MUC4, and therefore additional keratins or lineage-specific markers may be needed to exclude this possibility in some cases.
Quality
 IVD |  IVD |  IVD |  RUO |
Linkage
MUC4 Positive Control Slides, Product No. 406S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Legal Information
Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany
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Nicolas Moniaux et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(2), 253-261 (2004-01-20)
We have previously cloned the full-length cDNA (approximately 28 Kb) and established the complete genomic organization (25 exons/introns over 100 kb) of the human MUC4 mucin. This large molecule is predicted to protrude over 2 microm above the cell surface
N Moniaux et al.
British journal of cancer, 91(9), 1633-1638 (2004-10-21)
Mucins are members of an expanding family of large multifunctional glycoproteins. Pancreatic mucins have important biological functions, including the protection, lubrication, and moisturisation of the surfaces of epithelial tissues lining ductal structures within the pancreas. Several lines of evidence support
Suguru Yonezawa et al.
Journal of hepato-biliary-pancreatic sciences, 17(2), 108-124 (2009-09-30)
Mucins are high molecular weight glycoproteins that play important roles in carcinogenesis and tumor invasion. We have described, for the first time, that pancreatic ductal adenocarcinomas (PDACs) with an aggressive behavior and a poor outcome expressed MUC1 (pan-epithelial membrane-associated mucin)
Leona A Doyle et al.
The American journal of surgical pathology, 35(5), 733-741 (2011-03-19)
Low-grade fibromyxoid sarcoma (LGFMS) is a distinctive fibroblastic neoplasm that is characterized by alternating collagenous and myxoid areas, deceptively bland spindle cell morphology, a whorling architecture, and a t(7;16) translocation involving FUS and CREB3L2. Owing to variable morphology and a
Leona A Doyle et al.
The American journal of surgical pathology, 36(10), 1444-1451 (2012-09-18)
Sclerosing epithelioid fibrosarcoma (SEF) is a rare aggressive fibroblastic neoplasm composed of cords of epithelioid cells embedded in a dense collagenous stroma. The reported immunophenotype of SEF is nonspecific. Some SEF cases show morphologic and molecular overlap with low-grade fibromyxoid
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