137264

HEPES

EMPROVE^® EVOLVE

• Synonym(s): HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• MDL number: MFCD00006158
• UNSPSC Code: 12161700

Properties

• biological source: synthetic
• Quality Level: 400
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, microbial, and trace metals; tested
• pH: 4.7-5.3; 6.8-8.2
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• pKa: 7.5
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• storage temp.: 2-30°C
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable