55952
Fusaric acid
for HPLC derivatization, ≥99.0% (HPLC)
Synonym(s):
5-Butylpicolinic acid, 5-Butylpyridine-2-carboxylic acid
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About This Item
Empirical Formula (Hill Notation):
C10H13NO2
CAS Number:
Molecular Weight:
179.22
Beilstein:
125804
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.22
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grade
for HPLC derivatization
Quality Level
Assay
≥99.0% (HPLC)
technique(s)
HPLC: suitable
SMILES string
CCCCc1ccc(nc1)C(O)=O
InChI
1S/C10H13NO2/c1-2-3-4-8-5-6-9(10(12)13)11-7-8/h5-7H,2-4H2,1H3,(H,12,13)
InChI key
DGMPVYSXXIOGJY-UHFFFAOYSA-N
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General description
Fusaric acid is a novel proton-affinitive derivatizing agent, having an ionization moiety and a hydrophobic moiety. It is commonly used for the derivatization of alcohols and phenols, by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS).
Application
Fusaric acid may be used as a derivatizing reagent for the quantification of hydroxysteroids and dehydroepiandrosterone (DHEA) and sulfated DHEA in biological samples using liquid chromatography electrospray-ionization-tandem mass spectrometry (LC/ESI-MS/MS) technique.
Legal Information
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
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Chemical derivatization to enhance ionization of anabolic steroids in LC-MS for doping-control analysis
I. Athanasiadou et all.
TrAC, Trends in Analytical Chemistry, 42, 137-156 (2013)
Kouwa Yamashita et al.
Journal of the American Society for Mass Spectrometry, 21(2), 249-253 (2009-11-17)
A highly sensitive derivatization method for liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry of dehydroepiandrosterone (DHEA), testosterone (T), pregnenolone (P5), and 17alpha-OH-pregnenolone (17-OHP5) was developed based on the use of fusaric acid as a reagent. DHEA, P5, and 17-OHP5
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