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11427857910

Roche

Fluorescein-12-UTP

≥85% (HPLC), solution

Synonym(s):

Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)

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About This Item

Empirical Formula (Hill Notation):
C39H41N4O22P3
CAS Number:
134367-01-4
Molecular Weight:
1010.68
UNSPSC Code:
41116100

Quality Level

100

Assay

≥85% (HPLC)

form

solution

mol wt

1034.4

packaging

pkg of 25 μL (250 nmol; 10 mM)

manufacturer/tradename

Roche

storage temp.

−20°C

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General description

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.

Application

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jia-Yu Zhang et al.
Scientific reports, 10(1), 7429-7429 (2020-05-06)
Bioinformatic analysis reveals an enrichment of putative DNA:RNA hybrid G-quadruplex-forming sequences (PHQS) on both sides of the transcription start sites (TSSs) in the genome of warm-blooded animals, suggesting a positive selection of PHQSs in evolution and functional role of DNA:RNA
Gilbert Lauter et al.
Neural development, 6, 10-10 (2011-04-07)
In recent years, mapping of overlapping and abutting regulatory gene expression domains by chromogenic two-color in situ hybridization has helped define molecular subdivisions of the developing vertebrate brain and shed light on its basic organization. Despite the benefits of this

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