SCT250

BioTracker^™ Clickable Polyamine Spermine Live Cell Probe

• Synonym(s): BioTracker^™ Clickable Polyamine Spermine Live Cell Probe, clickable polyamine probe, live cell probe, polyamine probe, polyamine transport system probe, spermine probe
• UNSPSC Code: 41106514

Properties

• biological source: human
• Quality Level: 100
• packaging: vial of 500 μg
• manufacturer/tradename: Millipore
• technique(s): cell culture | stem cell: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

The relationship between polyamine systems and cancer---plus critical normal cell processes--- led to the creation of clickable polyamine derivatives which are innovative and novel tools for functional studies of the polyamine transport system (PTS). Spermidine and other important polyamine derivatives were conjugated to a BODIPY fluorophore through click chemistry.

MW: 743.35 g/mol

SPECTRAL PROPERTIES
λex: 494 nm
λem: 510 nm

Features and Benefits

The BioTracker Clickable Polyamine Spermine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine tracking with superior cellular uptake.Probe may be dissolved in DMSO to make a 10mM stock solution. 1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.

Target description

Polyamines play a variety of critical roles in the cell. Some of these processes include gene expression, cell proliferation and differentiation, and cellular stress management. Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal, ovarian, prostate, and pancreas. In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.

Storage and Stability

Store at -20 °C, protected from light

Other Notes

This spermine derivative click-conjugated to BODIPY is optiized for the study of the polyamine transport system, which is exploited in diverse cancers.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.