NS03L

Normal Mouse Serum

This Normal Mouse Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Mouse Serum.

• Synonym(s): Mouse Serum
• UNSPSC Code: 12352202
• NACRES: NA.43

Properties

• biological source: mouse
• form: lyophilized
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Normal carrier sera from healthy, non-immunized mice.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.

Reconstitution

Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable