MABN2656

Anti-Tau Antibody, clone 9B4

• Synonym(s): MAPTL, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), TAU
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 9B4, monoclonal
• mol wt: calculated mol wt 60 kDa; observed mol wt ~55 kDa
• purified by: using protein G
• species reactivity: rhesus macaque, human
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001116538
• UniProt accession no.: P10636
• storage temp.: 2-8°C
• Gene Information: human ... MAPT(4137)

Description

Specificity

Clone 9B4 is a mouse monoclonal antibody that detects Microtubule-associated protein tau. It targets an epitope within 36 amino acids from the N-terminal region of Microtubule-associated protein tau.

Immunogen

Recombinant human 1N3R isoform tau protein aggregates produced with arachidonic acid.

Application

Quality Control Testing

Evaluated by Western Blotting with His-tagged Human recombinant Tau, isoform 1N4R.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Human recombinant Tau isoform 1N4R.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Tau in Immunofluorescence application. (Kanaan, N.M., et al. (2021). Front Mol Neurosci. 14:607303).

Enzyme Immunoassay (ELISA): A representative lot detected Tau in ELISA application. (Kanaan, N.M., et al. (2021). Front Mol Neurosci. 14:607303).

Immunohistochemistry Applications: A representative lot detected Tau in Immunohistochemistry application. (Kanaan, N.M., et al. (2021). Front Mol Neurosci. 14:607303).

Western Blotting Analysis: A representative lot detected Tau in Western Blotting application. (Kanaan, N.M., et al. (2021). Front Mol Neurosci. 14:607303).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein Paired helical filament-tau (PHF-tau)) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau is a highly abundant protein found predominantly in the nervous system. Its primary role is to stabilize and support microtubule assembly, which are essential for maintaining the structure and function of nerve cells. It is present throughout the brain, particularly in regions involved in memory and cognition. MAPT gene consists of multiple exons that undergo alternative splicing, resulting in the generation of different isoforms that differ in the number and arrangement of repeats in the microtubule-binding domain. The expression of the MAPT transcripts varies and depends on the stage of neuronal maturation and neuron type. Aberrant aggregation and accumulation of tau protein have been linked to several neurodegenerative diseases collectively known as tauopathies. The most common tauopathy is Alzheimer′s disease, where hyperphosphorylated tau protein forms neurofibrillary tangles inside neurons. Other tauopathies include frontotemporal dementia, progressive supranuclear palsy, and cortico-basal degeneration. These diseases are characterized by cognitive decline, motor disturbances, and memory loss.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.