MAB3073
Anti-G Protein Goα Antibody, clone 2A
clone 2A, Chemicon®, from mouse
Synonym(s):
Anti-DEE17, Anti-G-ALPHA-o, Anti-GNAO, Anti-HG1G, Anti-HLA-DQB1, Anti-NEDIM
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2A, monoclonal
species reactivity
rat, bovine, human, guinea pig, mouse
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GNAO1(2775)
General description
The previously assigned protein identifier P29777 has been merged into P09471. Full details can be found on the UniProt database.
Specificity
Go-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have >95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also.
Immunogen
Bovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806).
Application
Anti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:3000.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide as a preservative.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt.
During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Quantitative confocal imaging of the retinal microvasculature in the human retina.
Tan, PE; Yu, PK; Balaratnasingam, C; Cringle, SJ; Morgan, WH; McAllister, IL; Yu, DY
Investigative Ophthalmology & Visual Science null
Interaction of the D2short dopamine receptor with G proteins: analysis of receptor/G protein selectivity.
Sarah A Nickolls, Philip G Strange, Sarah A Nickolls, Philip G Strange
Biochemical Pharmacology null
Bipolar cell diversity in the primate retina: morphologic and immunocytochemical analysis of a new world monkey, the marmoset Callithrix jacchus.
T L Chan, P R Martin, N Clunas, U Grunert
The Journal of Comparative Neurology null
Immunocytochemical evidence that monkey rod bipolar cells use GABA.
Lassova L, Fina M, Sulaiman P, Vardi N
The European Journal of Neuroscience null
Pavitra S Ramachandran et al.
Human gene therapy, 28(2), 154-167 (2016-10-19)
Within the next decade, we will see many gene therapy clinical trials for eye diseases, which may lead to treatments for thousands of visually impaired people around the world. To target retinal diseases that affect specific cell types, several recombinant
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service