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Key Documents

MAB1921P

Sigma-Aldrich

Anti-Laminin β1 Antibody, clone 4E10

clone 4E10, Chemicon®, from mouse

Synonym(s):

Laminin B1 Chain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4E10, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_002291.1

UniProt accession no.

P07942

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAMB1(3912)

Specificity

Reacts with the beta1 chain of intact laminin and blocks the neurite stimulating activity of human laminin. No cross-reactivity with mouse laminin.

Immunogen

Purified human laminin

Application

Detect Laminin β1 using this Anti-Laminin β1 Antibody, clone 4E10 validated for use in ELISA, IF, IP & WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western blot

Immunoprecipitation

Immunoflurescence

ELISA

Immunoaffinity chromatography

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.6, containing 0.1% sodium azide.

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis.
Wewer, U, et al.
The Journal of Biological Chemistry, 258, 12654-12660 (1983)
Increased laminin A expression in regenerating myofibers in neuromuscular disorders.
Mundegar, R R, et al.
Muscle and Nerve, 18, 992-999 (1995)
Jun Fan et al.
Frontiers in oncology, 11, 740410-740410 (2021-12-21)
Craniopharyngiomas (CPs) predominantly involving the third ventricle were commonly termed "intraventricular" lesions. The aim of this study was to clarify the anatomical relationship between the tumor and the third ventricle by both surgical and histological investigation. A retrospective review of
Enzyme immunoassay ELISA and EMIT.
E Engvall
Methods in enzymology, 70(A), 419-439 (1980-01-01)
Radhika Mhatre et al.
Annals of Indian Academy of Neurology, 24(2), 198-203 (2021-07-06)
Immunocharacterization of congenital muscular dystrophy (CMD) to determine the frequency of various subtypes in a large Indian Cohort. This retrospective (2014-2017) study was carried on muscle biopsies of clinically suspected cases of CMD with histological evidence of dystrophy/myopathic features. Immunohistochemistry
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