176695

Angeli′s Salt

A nitroxyl anion (NO⁻) donor that spontaneously dissociates in a pH dependent first order process to liberate 0.54 moles of NO per mole of the parent compound.

• Synonym(s): Angeli’s Salt, Sodium α-Oxyhyponitrite, Disodium Diazen-1-ium-1,2,2-triolate, AS, Sodium Trioxodinitrate
• Empirical Formula (Hill Notation): N₂O₃ · 2Na
• CAS Number: 13826-64-7
• Molecular Weight: 121.99
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥99%
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• solubility: alkaline solution: soluble (0.01 M NaOH)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A nitroxyl anion (NO^–) donor that spontaneously dissociates in a pH dependent first order process to liberate 0.54 moles of NO per mole of the parent compound. Half-life (t_½) in 100 mM phosphate buffer, pH 7.4 is 2.3 minutes at 37°C. Decomposition is nearly instantaneous at pH 5.0.

A nitroxyl anion (NO^-) donor that spontaneously, in the presence of oxygen and Cu²⁺ and Fe³⁺, dissociates in a pH-dependent, first-order process to liberate 0.54 moles of NO per mole of the parent compound. Half-life (t_½) of NO release = 2.3 min. (in 100 mM phosphate buffer, pH 7.4 at 37°C). Induces relaxation of norepinephrine-constricted rabbit aorta (EC₅₀ = 590 nM). Angeli′s Salt produces nitroxyl anion (NO^-) that can be converted to NO in the presence of heme.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Nitroxyl anion (NO-) donor

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution in alkaline solution, store at -20°C. This product is stable for 6 months as supplied. Alkaline stock solutions are stable for up to 24 h at -20°C. Unstable in acidic or neutral pH buffers.

Other Notes

Miranda, K.M., et al. 2001. J. Biol. Chem.276, 1720.
Hrinczenko, B.W., et al. 2000. Br. J. Haematol.110, 412.
Nelli, S., et al. 2000. Br. J. Pharmacol.131, 356.
Ma, X.L., et al. 1999. Proc. Natl. Acad. Sci. USA96, 14617.
Keefer, L.K., et al. 1996. Methods Enzymol.268, 281.
Fukuto, J.M., et al. 1993. Biochem. Biophys. Res. Commun.196, 707.
Maragos, C.M., et al. 1991. J. Med. Chem.34, 3242.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable