14-452-M

PDK1 Protein, active, 10 µg

Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• product line: Upstate^®
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol wt: M_w 59 kDa
• purified by: (Ni²⁺/NTA-agarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• accession no.: (FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. ); NM_002613.3
• NCBI accession no.: NM_002613.3; NM_031268.4
• UniProt accession no.: O15530
• Gene Information: human ... PDPK1(5170)

Description

General description

N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.

Biochem/physiol Actions

Protein Target: PDK1

Target Sub-Family: AGC

Packaging

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.

Quality

Routinely evaluated by phosphorylation of PDKtide

Target description

PDK1

Physical form

50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol

Ni²⁺/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(C): Not applicable