1.04157

Fluorescein-5-isothiocyanate (FITC)

• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• MDL number: MFCD00005063
• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• composition: Dye, ≥95% spectrophotometric assay
• Quality Level: 200
• mp: >360 °C
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.

Application

for biochemistry and for fluorescence microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable