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06-371

Sigma-Aldrich

Rabbit Anti-Mouse IgG Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

Specificity

mouse IgG

Immunogen

Highly purified whole mouse IgG (heavy and light chains)

Application

Rabbit anti-Mouse IgG Antibody detects level of Mouse IgG & has been published & validated for use in IP.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Quality

routinely evaluated by immunoprecipitating mouse IgG then blotting with anti-Fak (Catalog #05-182)

Target description

50-60kDa

Physical form

Format: Purified
Protein A chromatography
of 0.02M Potassium Phosphate, pH 7.2, 0.15M NaCl, with 0.01% sodium azide

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thomas A Milne et al.
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M Yamamoto et al.
The Journal of biological chemistry, 272(19), 12492-12494 (1997-05-09)
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M Pap et al.
The Journal of biological chemistry, 273(32), 19929-19932 (1998-08-01)
Growth factor-dependent survival of a variety of mammalian cells is dependent on the activation of phosphatidylinositol (PI) 3-kinase and its downstream effector, the protein kinase Akt. Glycogen synthase kinase-3 (GSK-3) has been previously identified as a physiological target of Akt

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