L040082

Cap A

• UNSPSC Code: 12352200
• NACRES: NA.21

Properties

• type: for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents

Description

Preparation Note

Acetic Anhydride / Tetrahydrofurane 9.1/90.9 (v/v)

Other Notes

Cap A solution, containing acetic anhydride and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Safety Information

• Pictograms: GHS02,GHS06,GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H225,H302,H315,H318,H331,H335,H336,H351
• Precautionary Statements: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH019
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 6.1 °F
• Flash Point(C): -14.4 °C