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606871

Sigma-Aldrich

ISOGRO®-15N Powder -Growth Medium

98 atom % 15N

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About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.12

isotopic purity

98 atom % 15N

Quality Level

technique(s)

bio NMR: suitable
protein expression: suitable

storage temp.

−20°C

Application

ISOGRO®-15N Powder -Growth Medium has been used:
  • for the production of 15N histone H2A for nuclear magnetic resonance (NMR) spectroscopy studies.
  • for the production of 15N-enriched protein kinase C and H3 histone for NMR studies.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Legal Information

ISOGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Expression and purification of the N-terminal regulatory domain of protein kinase C for biophysical studies
Cole TR and Igumenova TI
Protein Expression and Purification, 110(4), 14-21 (2015)
Mechanisms of ubiquitin-nucleosome recognition and regulation of 53BP1 chromatin recruitment by RNF168/169 and RAD18
Hu Q, et al.
Molecular Cell, 66(4), 473-487 (2017)
Carissa L Perez et al.
Cell metabolism, 8(3), 266-274 (2008-09-03)
Although studies in C. elegans have identified numerous genes involved in fat storage, the next step is to determine how these factors actually affect in vivo lipid metabolism. We have developed a (13)C isotope assay to quantify the contribution of
Xavier Hanoulle et al.
The Journal of biological chemistry, 282(47), 34148-34158 (2007-09-15)
The chemotaxis and integrin-mediated adhesion of T lymphocytes triggered by secreted cyclophilin B (CypB) depend on interactions with both cell surface heparan sulfate proteoglycans (HSPG) and the extracellular domain of the CD147 membrane receptor. Here, we use NMR spectroscopy to
Weizhi Liu et al.
The Journal of biological chemistry, 284(45), 31336-31349 (2009-08-28)
The eukaryotic translation initiation factor eIF4E recognizes the mRNA cap, a key step in translation initiation. Here we have characterized eIF4E from the human parasite Schistosoma mansoni. Schistosome mRNAs have either the typical monomethylguanosine (m(7)G) or a trimethylguanosine (m(2,2,7)G) cap

Articles

Our company presents an informative page regarding the use of ISOTEC Stable Isotopes to Amplify Recombinant Protein Expression with ISOGRO.

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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