HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano
CONDITIONS
column
Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U)
mobile phase
[A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B)
flow rate
1.5 mL/min
pressure
3270 psi (225 bar)
column temp.
30 °C
detector
UV, 225 nm
injection
5 μL
sample
20 μg/mL in mobile phase
Beschreibung
Hinweis zur Analyse
This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany