HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
Ascentis® Express Phenyl-Hexyl, 2,7 μm HPLC-Säule
2.7 μm particle size, L × I.D. 5 cm × 4.6 mmStandard
Produkt-Nr.
Beschreibung
Preisangaben
Alprazolam -Lösung
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam -Lösung
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam -Lösung
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase
[A] water; [B] acetonitrile; (62:38, A:B)
flow rate
1.4 mL/min
pressure
2600 psi (179 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/mL of each in 20% methanol
Beschreibung
Hinweis zur Analyse
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany