G005794

HPLC Analysis of Insulin (Species Variants) on BIOshell A160 Peptide C18

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U); mobile phase: [A] 70:30, water with 0.1% TFA:acetonitrile with 0.09% TFA; [B] 55:45, water with 0.1% TFA:acetonitrile with 0.09% TFA; gradient: 0 to 100% B in 7.5 min; flow rate: 0.3 mL/min; pressure: 4040 psi (278 bar); column temp.: 30 °C; detector: UV, 215 nm; injection: 0.5 μL; sample: 200 μg/mL insulin (bovine, human, porcine) in 0.1% formic acid
• Eignung: application for HPLC
• Anwendung(en): life science and biopharma; pharmaceutical (small molecule)

Beschreibung

Hinweis zur Analyse

These three insulins have more than 76% identical positions in a primary structure alignment. The human and porcine proteins are most similar with 85% identity. Baseline resolution of these insulins in achieved on BIOshell A160 Peptide C18, with relative band spacing of these three peaks approximating their relative sequence similarities. BIOshell A160 Peptide C18 is ideally suited for RP chromatography of small proteins or large peptides.

Rechtliche Hinweise

Analyt

Insulin from bovine pancreas, ≥25 USP units/mg (HPLC), powder
Insulin human, powder, recombinant, expressed in E. coli, suitable for cell culture
Insulin from porcine pancreas, powder, ≥27 USP units/mg