G005753

HPLC Analysis of an Energy Drink on Ascentis^® Express HILIC (UV Detection)

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U); mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C); flow rate: 0.6 mL/min; pressure: 812 psi (56 bar); column temp.: 35 °C; detector: UV, 254 nm; injection: 2 μL; sample: Dilute 1:9 in acetonitrile
• Eignung: application for HPLC
• Anwendung(en): food and beverages

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Caffeine
Benzoic acid
Sorbic acid