G003765

HPLC Analysis of Hydrocortisone in Topical Cream on Discovery^® HS C18 after SPE using Discovery^® DSC-Si

application for SPE, application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable; solid phase extraction (SPE): suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample addition: hydrocortisone topical hand cream in hexane:ethylacetate; SPE tube/cartridge: Discovery DSC-Si, 500 mg/3 mL (52695-U); condition: 1 mL hexane:ethyl acetate (2:1, v/v); sample addition: 1 mL; washing: 1 mL hexane:ethyl acetate (2:1, v/v); elution: 1 mL methanol:DI water (1:1, v/v); eluate post-treatment: dilute eluate to 2 mL with methanol:DI water (1:1, v/v); column: Discovery HS C18, 15 cm x 4.6 mm I.D., 5 μm particles (568520-U); mobile phase: [A] methanol; [B] DI water (1:1, A:B); flow rate: 1 mL/min; column temp.: 35 °C; detector: UV, 220 nm; injection: 10 μL
• Eignung: application for HPLC; application for SPE
• Anwendung(en): cleaning products; cosmetics; personal care; pharmaceutical (small molecule)

Beschreibung

Anwendung

Vorbehandlung der Probe:
Lösen von 1 g 1% Hydrocortison-Handcreme in 10 mL Essigester. Lösen von 2 mL der Essigester-Creme-probe in 8 mL Hexan:Essigester (2:1, v/v).

Konditionieren der SPE-Kartusche mit 1 mL Hexan:Essigester (2:1, v/v)
Aufgabe von 1 mL der vorbehandelten Probe
Auswaschen der lipophilen Störstoffe mit 1 mL Hexan:Essigester (2:1, v/v).
Eluieren mit 1 mL Methanol:DI H₂O (1:1, v/v)
Verdünnen des Eluats auf 2 mL mit Methanol:DI H₂O (1:1, v/v)

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany