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Merck

ZRB2312

Sigma-Aldrich

Anti-TRPV1 Antibody, clone 1N19 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(e):

Capsaicin receptor, OTRPC1, Osm-9-like TRP channel 1, Transient receptor potential cation channel subfamily V member 1, Vanilloid receptor 1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

1N19, recombinant monoclonal

Beschreibung

1N19 Clone

Produktlinie

ZooMAb® learn more

Form

lyophilized

Mol-Gew.

calculated mol wt 94.96 kDa
observed mol wt ~80 kDa

Aufgereinigt durch

using Protein A

Speziesreaktivität

mouse, monkey, human

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

Methode(n)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotyp

IgG

Epitopsequenz

C-terminal

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Angaben zum Gen

human ... TRPV1(7442)

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1N19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects TRPV1. It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal, cytoplasmic domain of human TRPV1.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected TRPV1 in COS-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TRPV1 in Human dorsal root ganglia tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TRPV1 in Neuro2A cells .

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TRPV1 in Human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound TRPV1 peptide with a binding affinity that was at least ten thousand-fold (10,000X) higher than that obtained with the non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Transient receptor potential cation channel subfamily V member 1 (UniProt: Q8NER1; also known as TrpV1, Capsaicin receptor, Osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1) is encoded by the TRPV1 (also known as VR1) gene (Gene ID: 7442) in human. TRPV1 is a multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It serves as a ligand-activated non-selective calcium permeant cation channel that is involved in detection of noxious chemical and thermal stimuli. It also mediates proton influx  and may be involved in intracellular acidosis in nociceptive neurons. It is also involved in mediation of inflammatory pain and hyperalgesia. Its channel activity is activated via the interaction with phosphoinositide interacting regulator of transient receptor potential channels (PIRT) and phosphatidylinositol 4,5-bisphosphate (PIP2). Both PIRT and PIP2 are required to activate channel activity. The channel is sensitized by ATP binding. Repeated stimulation of this channel with capsaicin leads to  progressively smaller responses, due to desensitization. This desensitization is triggered by the influx of calcium ions and is inhibited by elevated ATP levels. Higher expression of TRPV1 channel has been reported in several chronic inflammatory diseases, including chronic asthma, rheumatoid arthritis, osteoarthritis, and erosive esophagitis. However, TRPV1 deficiency has been associated with upregulation of proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6.  This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bujak, JK., et al. (2019). Front. Oncol. 9; 1087; Feng, J., et al. (2017). Nat. Commun. 8; 980).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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